Prep HPLC Column, Bidentate C8, 4um, 100A. 30mm ID x 100mm Length. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40008‑P30‑100 Cogent Bidentate C8 Preparative Column is designed for high‑load purification and preparative HPLC separations, utilizing 4 µm, 100 Å TYPE‑C™ silica hydride particles to deliver alternative selectivity, rapid equilibration, and long column lifetime under demanding conditions.
The Bidentate C8 stationary phase provides moderate hydrophobic retention with distinct selectivity compared to C18, making it ideal when:
C18 phases are too retentive
Faster elution and recovery are required
Compounds contain amine functionality
Larger fractions need to be collected
The 30 mm internal diameter (ID) classifies this column in the preparative / purification range, allowing high sample loading capacity and scalable separations.
Preparative Column Use‑Case Framework
100 mm Length – Higher Resolution Purification
Increased resolving power vs 50 mm columns
Better peak separation for complex mixtures
Ideal for compound isolation requiring purity and fraction integrity
Slightly higher backpressure and run time vs 50 mm
Flow Rate & System Requirements
30 mm ID preparative columns require specialized HPLC systems
Typical flow rate range: ~40 – 120 mL/min
Optimal starting range: ~50 – 80 mL/min for higher resolution
Requires:
Preparative HPLC system with high‑flow capability
Heavy‑duty pump heads and tubing
Fraction collection system for purification workflows
⚠️ Standard analytical HPLC systems cannot support this column size.
Flow rate optimization depends on:
Mobile phase composition and viscosity
Desired resolution vs throughput
System pressure limits
Sample load requirements
Why Choose Bidentate C8 for Preparative Work
Compared to traditional C18 phases, Bidentate C8:
Reduces excessive retention for hydrophobic compounds
Enables faster recovery of analytes
Provides alternative selectivity for polar and ionizable species
Improves recovery of amines and basic compounds
Enhances productivity in purification workflows
Cogent TYPE‑C™ Advantages
Silica Hydride Surface Technology
Low silanol activity
Improved peak shape for basic analytes
Reduced secondary interactions
Faster Equilibration
Shorter method cycle times
Increased throughput
Exceptional Durability
Long column lifetime
Stable under demanding prep conditions
Consistent performance over repeated runs
Typical Applications
Preparative purification of pharmaceuticals
Isolation of metabolites and reaction intermediates
Separation of amine‑containing compounds
High‑load reversed‑phase chromatography
Situations where C18 provides excessive retention
Made‑to‑Order & Pricing Information
This is a custom, made‑to‑order item.
Orders cannot be canceled once placed
Custom items are non‑returnable